Reference Links




*Courses*
Restricted Access

APC 285 - Stereology Course - 2005 APC 285 - Stereology Course - 2007	APC 286 - Microscopy Course - 2006



Technical References



Digital Imaging

Introduction to Digital Imaging from the Southwest Environmental Health Science Center (SWEHSC) Introduction to Optical Microscopy, Digital Imaging, and Photomicrography Digital Imaging: A Primer for Pathologists by Riley, et al. The Ethics of Digital Manipulation in Scientific Images by Hayden Formula for Deconvolution	How to Get the Optimal Resolution from Your CCD Camera Photometrics Digital Imaging Library Digital Photography article Hamamatsu PMT Spectral Response Hamamatsu PMT Construction Hamamatsu Application Notes Hamamatsu Technical Notes (includes a full PMT handbook)



Fluorochrome Data

Information and Brochures: Fluorophore Handbook (Molecular Probes) Information Regarding Alexa Fluor Dyes Alexa Fluor Succinimidyl Esters Alexa Fluor Dyes Brochure Optical Filters Handbook	Spectra Databases and Viewers: BD Biosciences: Spectra/Filter Viewer BioRad/Zeiss: Spectra/Filter Viewer Molecular Probes Fluorescence Spectra Database

Sample Spectra:
Antibody-bound Fluorochromes Alexa Fluor 488 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 568 Alexa Fluor 647 Texas Red FITC	DNA Detection Fluorochromes DAPI Propidium Iodide Ethidium Bromide Ethidium Homodimer-1



Microscopy

Bleaching Protection and Mounting Media Recipes for Certain Biological Stains Optical Microscopy by Abramowitz and Davidson Microscope Basics and Beyond by Abramowitz Microscopy from the Very Beginning Confocal Introduction (from Washington State University) Significance of the Pinhole in Confocal Microscopy Two-Photon Microscopy Fluorescence Lifetime Imaging	Comparison of Confocal Single-Mirror and Two-Mirror Point Scanners Phase Visualization as an Alternative to Ratio Imaging by Olschewski Factors Affecting Quantitative 3D Fluorescence Microscopy New Applications in Confocal Microscopy Using GFP Basic Principles of the C-DIC Method Explanation of PlasDIC Comparing and contrasting traditional DIC with PlasDIC



Organizations and Institutions

SPIE (The International Society for Optical Engineering) NIBIB (National Institute of Biomedical Imaging and Bioengineering) CBST (Center for Biophotonics, Science and Technology at UC Davis)	Jackson ImmunoResearch Laboratories (including a technical center) The Department of NanoBiophotonics for the Max Planck Society



Other Links, Pages and Forums

Fluorescent Probe Protocols Molecular Probes Links Page	SPIE Forum Page UK Surface Analysis Forum



Products and Equipment

Carl Zeiss Microscopy & Image Analysis Leica Microsystems Olympus Microscopes & Accessories Nikon Microscopes USA	Chroma Technology Corp. Molecular Probes Specialty Bulb Company QuantumDot



Software

Image Analysis Software including Confocal Assistant, Voxx, and Zeiss LSM offline browser	Using Confocal Assistant Open Microscopy Environment NIH Image (including a link to Image J)



Training, Resources, and Workshops

Olympus Microscopy Resource Center Confocal Microscopy for Biologists Nikon's Microscopy U Kodak Digital Learning Center Microscopy and Imaging Resources at SWEHSC Adobe Photoshop Tutorials	UMass Confocal Home Page CCD University (CCD Tutorials) Upcoming Courses and Workshops on Fluorescence Applications SPIE Continuing Education Program Zeiss Infomaterial for Laser Scanning Microscopes